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. 2011 Apr;193(8):1981–1990. doi: 10.1128/JB.01402-10

Table 2.

Thiol levels in wild-type M. smegmatis mc2155 and mshA mutants

Strain or genotype and growth phasea Mean level ± SD (μmol/g [dry wt], n = 3)
MSH Cysteine Coenzyme A Lipoic acidb Lipoamideb Ergothioneine
mc2155
    Exponential 8.8 ± 0.2 0.080 ± 0.005 1.8 ± 0.1 <0.001 <0.001 0.053 ± 0.01
    Stationary 2.4 ± 0.3 0.043 ± 0.004 1.2 ± 0.1 ND ND 0.11 ± 0.002
mshA::Tn5
    Exponential <0.003 0.082 ± 0.004 1.4 ± 0.1 <0.001 <0.001 1.4 ± 0.1
    Stationary <0.003 0.027 ± 0.001 1.0 ± 0.3 ND ND 0.52 ± 0.02
mshA(G32D)
    Exponential <0.003 0.095 ± 0.004 1.8 ± 0.2 <0.003 <0.003 1.85 ± 0.020
    Stationary <0.003 0.057 ± 0.007 1.2 ± 0.2 ND ND 0.85 ± 0.06
a

In the exponential phase, growth was at 37°C in liquid Middlebrook 7H9 medium, 1% glucose, 0.05% Tween 80 for 16 h (A600 ∼ 1). In the stationary phase, growth was as for exponential growth but for 48 h (A600 > 2.5).

b

Unbound forms. ND, not determined.

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