Table 1.
Strains used and constructed in this study
| Strain | Colony phenotype | Description | Reference |
|---|---|---|---|
| D39 | WT | S. pneumoniae serotype 2 | |
| D39:BCDΔ | Rough | cpsB, cpsC, and cpsD genes replaced with erm in D39, Ermr | 12 |
| D39:EHA-Cps2C | WT | erm inserted between cpsB and HA tagged cpsC, Ermr | This study |
| D39:EHA-I54T | Small | Ile→Thr substitution at residue 54 (note that all mutants were constructed in the D39:EHA-Cps2C background) | This study |
| D39:EHA-N60S | Mucoid | Asn→Ser substitution at residue 60 | This study |
| D39:EHA-Y82C | Mucoid | Tyr→Cys substitution at residue 82 | This study |
| D39:EHA-A91T | Small | Ala→Thr substitution at residue 91 | This study |
| D39:EHA-L92S | WT | Leu→Ser substitution at residue 92 | This study |
| D39:EHA-K106T | Mucoid | Lys→Thr substitution at residue 106 | This study |
| D39:EHA-V114A | Mucoid | Val→Ala substitution at residue 114 | This study |
| D39:EHA-E133G | Mucoid | Glu→Gly substitution at residue 133 | This study |
| D39:EHA-E144G | Mucoid | Glu→Gly substitution at residue 144 | This study |
| D39:EHA-A146T | Small | Ala→Thr substitution at residue 146 | This study |
| D39:EHA-V159E | Mucoid | Val→Glu substitution at residue 159 | This study |
| D39:EHA-R166D | Small | Arg→Asp substitution at residue 166 | This study |
| D39:EHA-P167G | Small | Pro→Gly substitution at residue 167 | This study |
| D39:EHA-A168T | Small | Ala→Thr substitution at residue 168 | This study |
| D39:EHA-P171G | Small | Pro→Gly substitution at residue 171 | This study |
| D39:EHA-S172R | Small | Ser→Arg substitution at residue 172 | This study |
| D39:EHA-S173R | WT | Ser→Arg substitution at residue 173 | This study |
| D39:EHA-P174G | Small | Pro→Gly substitution at residue 174 | This study |
| D39:EHA-N175F | WT | Asn→Phe substitution at residue 175 | This study |
| D39:EHA-L181W | Small | Leu→Trp substitution at residue 181 | This study |
| D39:EHA-G183A | WT | Gly→Ala substitution at residue 183 | This study |
| D39:EHA-G186A | Small | Gly→Ala substitution at residue 186 | This study |
| D39:EHA-G187A | Small | Gly→Ala substitution at residue 187 | This study |
| D39:EHA-D202Δ | Rough | HA-cps2C truncated after residue 202 | This study |