Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
MicF-dependent reduction of OmpF expression in tolC mutants is a result of Rob activation of micF gene expression. Cells were grown overnight in medium A and subcultured 1:200 in fresh medium A containing 5 mM salicylate. Cultures were grown to mid-logarithmic phase prior to envelope extraction. Envelope fractions were displayed on 10% acrylamide–6 M urea-1% SDS gels and stained with Coomassie R250. Strains used were MG1655, CR702, and CR732 to CR736.
