Fig. 3.

RT-PCR amplification of the hrdB (a) and afsS (b) transcripts using the RNA of S. coelicolor M145 (parental strain, lane 1), INB201 (ΔphoP mutant, lane 2), M513 (ΔafsR mutant, lane 3), and INB513 (ΔafsR ΔphoP mutant, lane 4) strains. Cultures were grown in MG-3.2 medium, and samples for RNA were taken at 44 h. The hrdB transcript is used as an expression control. For the DNA control reactions (represented by C1 to C4), Platinum Taq was added instead of retrotranscriptase. Lane M, molecular size marker.