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. 2011 May;193(9):2133–2140. doi: 10.1128/JB.01350-10

Fig. 1.

Fig. 1.

Yeast two-hybrid results showing interaction between YirB and YjbH proteins. (A) Schematic of plates shown in panel B. AD denotes activation domain fusion, and BD is the DNA-binding domain fusion. (B) Yeast strains bearing two-hybrid constructs. The plate on the left contains Trp− Leu− Ade− dropout medium; growth without Ade is indicative of YirB-YjbH fusion interaction. On the right is a control plate that selects for two hybrid plasmid-bearing strains. No evidence of interaction is observed when a control BD plasmid or a BD fusion construct made with the N-terminal deletion derivative of YjbH, YjbH(Δ1–24), is propagated in the yeast strain carrying the YirB-AD fusion plasmid. (C) Organization of the chromosomal region of B. subtilis where the yirB (yuzO) gene resides. The black arrow indicates the location and transcriptional orientation of yirB. The yirB gene resides downstream of the cssRS operon (white arrows) encoding the two-component regulatory system controlling the cell's response to secretion stress. Also shown are the location and transcriptional orientation of the htrB gene that is divergently transcribed from cssRS.