Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype |
|---|---|
| Strains | |
| JH642 | trpc2 pheA1 |
| ORB3834 | trpc2 pheA1 spx::neo |
| ORB6824 | trpc2 pheA1 thrC::trxB-lacZ |
| ORB4541 | trpC2 pheA1 trxB::trxB-lacZ |
| ORB4561 | trpC2 pheA1 trxB::trxB-lacZ spx::tet |
| ORB7399 | trpC2 pheA1 amyE::pSK1 (Physpank-yirB) |
| ORB7402 | trpC2 pheA1 amyE::Physpank |
| ORB7403 | trpC2 pheA1 spx::neo amyE::pSK1 (Physpank-yirB) |
| ORB7406 | trpC2 pheA1 spx::neo amyE::Physpank |
| ORB7407 | trpC2 pheA1 thrC::trxB-lacZ amyE::pSK1 (Physpank-yirB) |
| ORB7408 | trpC2 pheA1 thrC::trxB-lacZ amyE::Physpank |
| ORB7430 | trpC2 pheA1 ΔyirB::ermf |
| ORB7431 | trpC2 pheA1 ΔyirB::ermr |
| ORB7437 | trpC2 pheA1 ΔyirB::ermf trxB::trxB-lacZ |
| ORB7556 | trpC2 pheA1 ΔyirB::erm amyE::Physpank-yirB trxB::trxB-lacZ |
| ORB7557 | trpC2 pheA1 ΔyirB::erm amyE::Physpank-yirB-His6trxB::trxB-lacZ |
| Plasmids | |
| pDR111 | Physpank spc bla |
| pUC19 | A derivative of pUC18 |
| pTKlac | Promoter-probe vector; Ampr Cmr |
| pDG646 | Source of erythromycin resistance cassette |
| pSK1 | Phyperspank fused with yirB gene |
| pSK5.1, 5.2 | pUC18 with yirB::erma |
| pSG-3 | yjbH coding sequence in yeast two-hybrid vector pGBKT7 |
| pSG-4 | yjbH(Δ1–24) coding sequence in yeast two-hybrid vector pGBKT7 |
a
erm, erythromycin-resistant marker.