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In A. salmonicida, loss of GspAAs abrogates assembly of the GspD secretin multimer. Anti-GspDAh immunoblot analysis of 0.5- and 5-fold-concentrated whole-cell samples taken from AsR and As-gspA strains grown in liquid culture to an OD600 of 2.0 is shown. Locations of prestained standard protein markers (in kDa) and the GspDAs secretin multimer are given.
