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Secretin assembly in A. hydrophila gspAB and the wild type upon expression of V. cholerae gspAB in trans. The amount of secretin multimer assembled upon induced expression (0.1 mM IPTG) of gspABAh, gspABVc, or neither (pMMB207) in C5.84 (A) and Ah65 (B) was assessed by anti-GspDAh immunoblot analysis. Locations of prestained standard protein markers (in kDa) are given.
