Table 5.
Nodulation of Lablab purpureus, Leucaena leucocephala, Tephrosia vogelii, and Vigna unguiculata by NGR234 and derivative mutants
| Plant (nodule type)a and strain | Mean ± SDb |
||
|---|---|---|---|
| No. of nodules | Nodule wt (mg) | Dry wt (mg) | |
| Lablab purpureus (D) | |||
| Control | 0 | 0 | 700 ± 190 |
| NGR234 | 10 ± 4 | 520 ± 160 | 1,020 ± 280 |
| NGRΔacpXL | 0* ± 0 | 0* ± 0 | 650* ± 140 |
| NGRΩbacA | 10 ± 5 | 450 ± 220 | 1,020 ± 350 |
| Leucaena leucocephala (I) | |||
| Control | 0 | 0 | 90 ± 15 |
| NGR234 | 15 ± 3 | 140 ± 30 | 240 ± 40 |
| NGRΔacpXL | 11 ± 3 | 130 ± 20 | 230 ± 50 |
| NGRΩbacA | 13 ± 2 | 140 ± 60 | 240 ± 90 |
| Tephrosia vogelii (I) | |||
| Control | 0 | 0 | 150 ± 30 |
| NGR234 | 5 ± 4 | 200 ± 110 | 260 ± 100 |
| NGRΔacpXL | 0* ± 0 | 0* ± 0 | 150* ± 30 |
| NGRΩbacA | 4 ± 2 | 120 ± 80 | 200 ± 70 |
| Vigna unguiculata (D) | |||
| Control | 0 | 0 | 90 ± 20 |
| NGR234 | 80 ± 18 | 740 ± 150 | 1,320 ± 270 |
| NGRΔacpXL | 0* ± 0 | 0* ± 0 | 60* ± 10 |
| NGRΩbacA | 85 ± 28 | 820 ± 130 | 1,240 ± 350 |
a
D, determinate nodules; I, indeterminate nodules.
b
The figures listed are per plant. Only pink (i.e., functional) nodules were scored. Nodulation tests were repeated at least twice, with 6 to 8 plants being tested each time. Statistical analyses (Student's t test) were performed to compare each mutant to the wild-type strain. *, significant difference (P ≤ 0.01).