Fig. 5.
Commitment of spores of various B. subtilis strains to germinate. (A and B) Heat-activated spores of strains PS533 (wild-type) and FB62 (gerD) (A) and PS3411 (↑SpoVA), PS3640 (spoVA1), and PS3642 (spoVA2) (B) germinated at 30°C in response to l-valine, and the kinetics of commitment (open symbols) and CaDPA release (filled symbols) were measured as described in Materials and Methods. (A) Kinetics of commitment (white symbols) and release of CaDPA (black symbols) in B. subtilis PS533 (wild-type) spores (circles) and FB62 (gerD) spores (triangles). (B) Kinetics of commitment (white symbols) and release of CaDPA (black symbols) in B. subtilis PS533 (wild-type) spores (circles), PS3411 (↑SpoVA) spores (squares), PS3640 (spoVA1) spores (triangles), and PS3642 (spoVA2) spores (diamonds).