Table 3.
Parameters of the germination of spores of various B. subtilis strains at 25°C and 45°C in response to the addition of dodecylaminea
| Germination temp (°C) | Strain | Tlag (min) | T1 (min) | Trelease (min) | ΔTrelease (min) | Δ(T1 − Tlag) (min) | Δ(Trel − T1) (min) | Ilag (%) | I1 (%) | Irelease (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| 25 | PS533 (wild type) | 48 ± 30.4 | 50.4 ± 30.9 | 51.3 ± 30.9 | 3.3 ± 1.6 | 2.4 ± 1.6 | 0.9 ± 0.2 | 90 ± 3 | 69 ± 1 | 18 ± 6 |
| FB62 (gerD) | 48.0 ± 32.0 | 50.9 ± 32.5 | 51.8 ± 32.6 | 3.8 ± 1.9 | 2.9 ± 1.8 | 0.9 ± 0.3 | 90 ± 3 | 69 ± 8 | 17 ± 4 | |
| PS3411 (↑SpoVA) | 43.6 ± 26.5 | 46.2 ± 27.1 | 47 ± 27.1 | 3.4 ± 1.0 | 2.5 ± 1.7 | 0.9 ± 0.2 | 89 ± 6 | 62 ± 11 | 17 ± 4 | |
| PS3640 (spoVA1) | 53.1 ± 31.7 | 56.1 ± 32.5 | 57 ± 32.6 | 3.9 ± 2.0 | 3.0 ± 1.9 | 0.9 ± 0.2 | 90 ± 4 | 65 ± 17 | 15 ± 4 | |
| PS3642 (spoVA2) | 49 ± 32.6 | 52.5 ± 33.4 | 53.7 ± 33.4 | 4.4 ± 3.1 | 3.5 ± 3.0 | 0.9 ± 0.3 | 89 ± 4 | 64 ± 14 | 18 ± 6 | |
| 45 | PS533 (wild type) | 16.3 ± 15.3 | 18.6 ± 15.59 | 17.8 ± 15.6 | 2.3 ± 1.3 | 1.5 ± 1.3 | 0.8 ± 0.2 | 90 ± 4 | 66 ± 13 | 16 ± 5 |
| FB62 (gerD) | 9.9 ± 7.0 | 11.4 ± 7.7 | 12.3 ± 7.7 | 2.4 ± 1.1 | 1.6 ± 1.0 | 0.9 ± 0.2 | 93 ± 4 | 66 ± 17 | 12 ± 3 | |
| PS3411 (↑SpoVA) | 7.26 ± 5.3 | 8.6 ± 6 | 9.4 ± 6.1 | 2.2 ± 1.2 | 1.4 ± 1.1 | 0.8 ± 0.2 | 92 ± 4 | 70 ± 13 | 19 ± 6 | |
| PS3640 (spoVA1) | 15.7 ± 15.0 | 19.3 ± 15.3 | 20.3 ± 15.3 | 4.6 ± 2.8 | 3.6 ± 2.7 | 0.9 ± 0.2 | 91 ± 4 | 54 ± 12 | 16 ± 5 | |
| PS3642 (spoVA2) | 14.7 ± 9.8 | 19.1 ± 12.1 | 20.2 ± 12.2 | 5.5 ± 4.5 | 4.4 ± 4.5 | 1.1 ± 0.4 | 90 ± 9 | 61 ± 19 | 16 ± 5 |
Spores of various B. subtilis strains germinated at 25°C or 45°C in response to dodecylamine. The parameters of the germination process were determined for 30 individual spores for each strain by DIC microscopy as described in Materials and Methods, and the values were averaged and standard deviations were calculated. The various kinetic parameters of spore germination are defined as follows. Tlag is the time at which rapid CaDPA release began. T1 is the time at which the rate of CaDPA release following Tlag shifted to an even faster rate. Trelease is the time at which CaDPA release was complete. ΔTrelease is Trelease − Tlag. Ilag is the normalized DIC intensity at Tlag. I1 is the normalized DIC intensity at T1. Irelease is the normalized DIC intensity at Trelease.