Table 2.

Bicyclomycin-resistant single mutants^a

Strain	MIC (μg/ml)	Gene production description
BW25113	30
hfq mutant	>50	RNA chaperone facilitating sRNA-mRNA pairing interactions
acs mutant	50	Acetyl-coenzyme A synthetase (AMP forming)
proW mutant	50	Proline ABC transporter
rseB mutant	50	Anti-sigma factor
efeB mutant	50	Peroxidase; cryptic ferrous iron transporter
trmU mutant	50	tRNA methyltransferase
fliG mutant	50	Flagellar motor switch protein
flgI mutant	50	Flagellar P-ring protein
hinT mutant	50	Purine nucleoside phosphoramidase
ynfA mutant	50	Inner membrane protein
adhE mutant	45	Alcohol/acetaldehyde dehydrogenase
caiA mutant	45	Crotonobetainyl-coenzyme A reductase
ydjE mutant	45	MFS transporter
rhlE mutant	45	ATP-dependent RNA helicase
galE mutant	45	UDP-glucose 4-epimerase
yciU mutant	45	Predicted protein
yciS mutant	45	Conserved inner membrane protein
gloB mutant	40	Glyoxalase II
hybD mutant	40	Maturation peptidase for hydrogenase 2
yneJ mutant	40	Predicted DNA-binding transcriptional regulator, LysR type
greA mutant	40	Transcription elongation factor
lysS mutant	40	Lysyl-tRNA synthetase
ydgA mutant	40	Conserved membrane protein
ygdD mutant	40	Conserved inner membrane protein
ymfJ mutant	40	e14 prophage
seqA mutant	40	Negative modulator of replication initiation
poxA mutant	40	Putative regulator of pyruvate oxidase

^aGene knockouts are from the KEIO collection, using BW25113 as the starting strain.