Table 1.
Catalytic parameters of DadA
| Substrate | kcat (s−1)a | Km (mM)a | kcat/Km (M−1 s−1) |
|---|---|---|---|
| d-Alanine | 0.74 ± 0.02 | 0.46 ± 0.05 | 1.61 × 103 |
| d-Histidine | 0.84 ± 0.01 | 0.81 ± 0.07 | 1.04 × 103 |
| d-Phenylalanine | 1.10 ± 0.06 | 1.07 ± 0.22 | 1.03 × 103 |
| d-Tyrosine | 1.10 ± 0.09 | 1.78 ± 0.49 | 6.18 × 102 |
| d-Proline | 0.97 ± 0.03 | 1.82 ± 0.21 | 5.33 × 102 |
| d-Serine | 0.80 ± 0.02 | 1.65 ± 0.15 | 4.85 × 102 |
| d-Threonine | 0.68 ± 0.02 | 2.47 ± 0.18 | 2.75 × 102 |
| d-Valine | 0.78 ± 0.03 | 3.01 ± 0.29 | 2.59 × 102 |
| d-Arginine | 1.27 ± 0.07 | 5.37 ± 0.69 | 2.36 × 102 |
a
The values represent the averages of two measurements ± SD.