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Thermal denaturation of HBHA constructs monitored at 222 nm. (a) Constructs 6_199 (●), 1_199 (■), 6_156(▴), and 6_111 (♦) were all at 10 μM. (b) Constructs 6_156 (▴), 6_138 (■), 6_111 (♦), and 6_88 (●) were all at 10 μM. (c) Plot of construct size (number of amino acids) versus calculated melting temperature.
