Expression analysis of transcriptional fusions of the hpxBASC-guaD gene cluster in different genomic backgrounds and under different growth conditions. Cells of the parental strain KC2653 (black bars) and the derived hpxS (white bars), nac (dashed bars), or ntrC (gray bars) mutant strain bearing the promoter fusion Φ(hpxSAB-lacZ), Φ(hpxC-lacZ), or Φ(guaD-lacZ) were grown in the indicated culture media. GNGln is a nitrogen excess medium that contains 0.4% glucose and 0.2% each ammonium sulfate and Gln, GGln is a nitrogen-limiting medium that contains 0.4% glucose and 0.04% Gln, and GGlnAll is the nitrogen-limiting medium containing 0.05% allantoin. β-Galactosidase activity is expressed in units/mg. Error bars indicate standard deviations.