Fig. 3.

Effects of the gerAA107 and gerAA111 mutant alleles on loss of heat resistance and DPA release during germination. Spores germinating at 37°C in 10 mM Tris-HCl (pH 7.5) containing 1 mM l-alanine and 20 mM KCl were sampled at intervals, and loss of heat resistance and release of DPA were measured. Values for the loss of heat resistance (black symbols) and release of DPA (white symbols) in strain 1604 (ger⁺) (circles), gerA111 mutant (triangles), and gerAA107 mutant (squares) are shown. The percentages of OD loss at 60 min were 27 for the gerAA111 mutant and 20 for the gerAA107 mutant.