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. 2011 May;193(9):2268–2275. doi: 10.1128/JB.01398-10

Fig. 5.

Fig. 5.

Levels of GerAC protein in gerAA mutant spores. Western blot of gerAA mutant broken spore extracts were separated on a Nu-PAGE gel (Invitrogen), probed with anti-GerAC antibody, and detected using ECL Plus (GE Healthcare). The GerAC band runs at 42 kDa under the conditions used. (A) Lanes: 1, MagicMark protein markers (Invitrogen); 2, strain 1604; 3, gerA84 mutant; 4, gerA16 mutant; 5, gerA80 mutant; 6, gerAA105 mutant; 7, gerAA106 mutant; 8, gerAA109 mutant; 9, gerAA114 mutant; 10, gerAA115 mutant. (B) Lanes: 1, strain 160; 2, gerAA108 mutant; 3, gerAA109 mutant; 4, gerA56 mutant; 5, gerAA84 mutant; 6, gerAA114 mutant; 7, gerAA115 mutant.