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. 2011 May;193(9):2268–2275. doi: 10.1128/JB.01398-10

Table 1.

Mutant strains used in this studya

Strain gerAA alleleb AA changeb Mutation type(s)c Phenotypic groupd GerAC statuse
BH4951 80 E129K R III Present
AM1671 108 N146A D III None
AM1666 103 V252A D I
AM1667 104 S294P D II
AM1672 109 H304A D III None
AM1673 110 P324S D I*
AM1674 111 F325A D I
AM1681 113 P326S D
AM1683 114 E330A D III None
AM1684 115 E335A D III 5%
BH4819 7 P349S R II
AM1878 84 V369 R, FS III None
AM1668 105 L373F D III Present
BH4818 16 G398S R III Present
AM1675 112 L399N D II
BH4810 56 S400F R III Present
AM1669 106 M409N D III Present
AM1670 107 H430A D II
a

Strains from the mutant collection of D. A. Smith contained random mutations. Strain AM1681 had phase-dark spores, and strain BH4818 was temperature sensitive.

b

The gerAA allele and the consequent amino acid (AA) change are shown.

c

D, site-directed mutation; R, random mutation; FS, frameshift mutagenesis.

d

The phenotypic groups are based on l-alanine-dependent germination. Group I strains showed normal or near-normal germination. The group I* strain was more sensitive to germinant. Group II strains showed a slower decrease in the optical density, while group III strains showed little or no response to germinant.

e

The GerAC status is shown only for group III mutants and reports the presence or absence and the level of GerAC protein detected in Western blots of spore extracts.