Table 1.
Strain | gerAA alleleb | AA changeb | Mutation type(s)c | Phenotypic groupd | GerAC statuse |
---|---|---|---|---|---|
BH4951 | 80 | E129K | R | III | Present |
AM1671 | 108 | N146A | D | III | None |
AM1666 | 103 | V252A | D | I | |
AM1667 | 104 | S294P | D | II | |
AM1672 | 109 | H304A | D | III | None |
AM1673 | 110 | P324S | D | I* | |
AM1674 | 111 | F325A | D | I | |
AM1681 | 113 | P326S | D | ||
AM1683 | 114 | E330A | D | III | None |
AM1684 | 115 | E335A | D | III | 5% |
BH4819 | 7 | P349S | R | II | |
AM1878 | 84 | V369 | R, FS | III | None |
AM1668 | 105 | L373F | D | III | Present |
BH4818 | 16 | G398S | R | III | Present |
AM1675 | 112 | L399N | D | II | |
BH4810 | 56 | S400F | R | III | Present |
AM1669 | 106 | M409N | D | III | Present |
AM1670 | 107 | H430A | D | II |
Strains from the mutant collection of D. A. Smith contained random mutations. Strain AM1681 had phase-dark spores, and strain BH4818 was temperature sensitive.
The gerAA allele and the consequent amino acid (AA) change are shown.
D, site-directed mutation; R, random mutation; FS, frameshift mutagenesis.
The phenotypic groups are based on l-alanine-dependent germination. Group I strains showed normal or near-normal germination. The group I* strain was more sensitive to germinant. Group II strains showed a slower decrease in the optical density, while group III strains showed little or no response to germinant.
The GerAC status is shown only for group III mutants and reports the presence or absence and the level of GerAC protein detected in Western blots of spore extracts.