Table 2.
Inhibition of germination of wild-type, gerAA110, and gerAA111 spores by d-alanine a
| Ratio of alanine isomers (l-alanine/d-alanine) | Alanine isomer concns (mM) (l-alanine/d-alanine) | Inhibition (%) |
||
|---|---|---|---|---|
| Wild type | AA110 mutant | AA111 mutant | ||
| 1:1 | 1:1 | >95 | 37 | 88 |
| 2:1 | 1:0.5 | 62 | 12 | 78 |
| 4:1 | 1:0.25 | 24 | <5 | 53 |
| 8:1 | 1:0.125 | 8 | <5 | 31 |
| 10:1 | 1:0.1 | <5 | <5 | 28 |
| 20:1 | 1:0.05 | <5 | <5 | 16 |
a
All conditions contained 20 mM KCl in 10 mM Tris-HCl (pH 7.5). Germination was measured by the loss of optical density (OD), and the percentage of inhibition was calculated as 100 × [(maximum rate of the decrease in the OD in the presence of d-alanine)/(maximum rate of the decrease in OD in 1 mM l-alanine only)]. Germination was not seen in d-alanine in the absence of l-alanine (OD decrease of ≤5% in 120 min).