Table 3.
Sensitivity of RL3499-RL3502 mutant strains to glycine, detergents, and alkaline pH
| Strain | Log growth reduction (mean ± SD)a |
||||
|---|---|---|---|---|---|
| TY | VMM | VMM + Gly | VMM + SDS | VMM, pH 9.0 | |
| 3841 | −0.09 ± 0.13 | 0 | −0.11 ± 0.14 | 0.79 ± 0.19 | 0.06 ± 0.25 |
| SM1 | >7.0 | 0 | 4.8 ± 0.19 | 5.9 ± 0.03 | 3.7 ± 1.0 |
| 38EV99 | >7.0 | 0 | 4.0 ± 0.36 | 3.9 ± 0.15 | 2.5 ± 1.3 |
| 38EV00 | >7.0 | 0 | 4.4 ± 0.23 | 4.0 ± 0.12 | 3.2 ± 0.30 |
| 38EV01 | >7.0 | 0 | 4.4 ± 0.28 | 4.0 ± 0.19 | 3.0 ± 0.53 |
| 38EV02 | >7.0 | 0 | 4.5 ± 0.11 | 4.4 ± 0.16 | 3.1 ± 0.42 |
| 38EV87 | >7.0 | 0 | 5.0 ± 0.30 | 5.3 ± 0.14 | 6.1 ± 0.50 |
a
Difference in log CFU/ml between VMM and VMM with 1 mM glycine (Gly) or 0.35 mM SDS or difference in log CFU/ml between VMM at pH 7.0 and VMM at pH 9.0 (see Materials and Methods). All nonzero values for the mutants represent statistically significant differences between the wild type and mutant strains at a P value of <0.005 (Student's t test).