Fig. 3.

MrpC2 and FruA bind to a downstream positive regulatory element. (A) Binding of MrpC2 and FruA to site 3. EMSAs with ³²P-labeled fmgE DNA (2 nM) spanning from position −25 to the indicated 3′ ends and His₁₀-MrpC2 (1 μM), FruA-His₆ (3 μM), or both His₁₀-MrpC2 (1 μM) and FruA-His₆ (3 μM), as indicated. The filled arrowhead points to the shifted complex produced by His₁₀-MrpC2 alone, and the open arrowhead points to the complex produced by FruA-His₆ alone. (B) Effects of 3′-end deletions on developmental fmgE-lacZ expression. β-Galactosidase specific activity during development was measured for lacZ fused to fmgE spanning from positions −100 to +50 (▴), −100 to +93 (○), or −100 to +116 (■). The units of activity are nanomoles of o-nitrophenyl phosphate per minute per milligram of protein. Points show the average values of three transformants, and each error bar depicts 1 standard deviation of the data.