Table 2.
Primers used in this study
| Primer | Sequencea | Description or referenceb |
|---|---|---|
| LK0926 | GGCTCGAGGTTTTTCTCTGTGAAAGAGGAC | Position −150 forward with XhoI site |
| LK1034 | CCGGATCCGTTGTTACCGGCATTGGTGC | Position +50, reverse with BamHI site |
| LK1245 | CCGGATCCACTGGCGCCGGCTGTAGGC | Position +251, reverse with BamHI site |
| LK1328 | GGCTCGAGCATTCTGTGCGGCGTTTCAG | Position −100, forward with XhoI site |
| LK1861 | CCTTGAGCGCGATGGAGATA | 60 |
| LK1862 | CTCGGCGGCCTCATCGAC | 60 |
| LK2442 | GAGGGCGCACGAATCGTCC | Position −25, reverse |
| LK2464 | CAATCCCATGTCCTCATCTG | Position −25, forward |
| LK2487 | TTTCTCTGTGAAAGAGGACCC | Position −147, forward |
| LK2512 | CCTGAAACGCCGCACAGAATG | Position −80, reverse |
| LK2527 | GGATCCCGATTGCGTTCCTGCTCCATG | Position +116, reverse with BamHI site |
| LK2577 | GGCTCGAGCATTAACGGGCGATCTTCCTC | Position −120, forward with XhoI site |
| LK2578 | GGCTCGAGCGATCTTCCTCATTCTGTGCG | Position −110, forward with XhoI site |
| LK2606 | GGATCCGGTGAGGACAGCGGTCAG | Position +93, reverse with BamHI site |
a
Restriction sites are underlined.
b
The position number is relative to the start site of fmgE transcription, and the orientation (forward or reverse) is relative to the direction of fmgE transcription.