Table 3.
Transcription from the srpA promoter in the presence or absence of toluene in srpS or srpR deletion backgrounds compared to the wild-type background
| P. putida strain | β-Galactosidase activity (Miller units)a |
|
|---|---|---|
| Without toluene | With toluene | |
| S12 lacZ strain | 892 ± 13 | 5,023 ± 363 |
| SrpS−lacZ strain | 4,500 ± 223 | 4,528 ± 102 |
| SrpR−lacZ strain | 153 ± 7 | 1,555 ± 95 |
a
β-Galactosidase assays were performed in the srpA-lacZ reporter SrpS− lacZ, SrpR− lacZ, and S12 lacZ gene fusion strains in the presence or absence of 6 mM toluene. The values in the table are the averages of at least three independent assays followed by the standard deviations.