Fig. 3.

Consumption of chemically generated NO· by the wild-type and mutant strains of M. catarrhalis O35E. Cell suspensions of the wild-type strain, the norB mutant, the nsrR norB mutant, and the aniA norB mutant were exposed to 20 μM NO· produced by the addition of 10 μM proline nitric oxide (Proli-NO). The dissolved NO· concentration was monitored using an NO· -specific electrode. For reference, the NO· -consuming activity of BHI medium was determined (data not shown). This experiment was performed three times, and representative data are shown.