Fig. 4.

Expression of AniA protein in wild-type and mutant strains of M. catarrhalis O35E. Whole-cell lysates were probed by Western blot analysis with polyclonal AniA antiserum (A) or with the CopB-specific MAb 10F3 (26) (B) as the primary antibody. Lane 1, wild-type O35E; lane 2, O35E norB; lane 3, O35E norB aniA; lane 4, O35E norB nsrR. The position of the putative AniA monomers is indicated by an arrow on the right side of panel A. The CopB outer membrane protein was used as a loading control, and its position is indicated by an arrow on the right side of panel B. Molecular weight position markers (in thousands) are present on the left side of each panel.