Fig. 3.
Localization of OspC mutants. (A) Proteinase K (pK) accessibility immunoblots of OspC tether mutants compared with that of OspAwt. FlaB was used as a periplasmic, protease-resistant control. (B) Membrane fractionation immunoblots of proteinase K-resistant, i.e., periplasmic OspC tether mutants compared with that of OspAwt. OppAIV served as the IM control. OMV, outer membrane vesicle fraction; PC, protoplasmic cylinder fraction (also containing intact cells) (59, 64). The OMV ratio was calculated from densitometry data that were normalized to both OspA and OppAIV as described previously (31). An asterisk (*) in both panels indicates a CtpA-dependent OspC band (see text) (43) (Kumru et al., submitted).