Table 1.
Bacterial strains and plasmids used in this study
| Strain/plasmid | Description | Source/reference |
|---|---|---|
| Strains | ||
| Borrelia burgdorferi | ||
| B313 | Clone of B31 ATCC 35210 (cp26, cp32-1, cp32-2/7, cp32-3, and lp17) | 55 |
| B31e2 | Clone of B31 ATCC 35210 (cp26, cp32-1, cp32-3, cp32-4, lp17, lp38, and lp54) | 3 |
| B31-A3 (ΔospC) | Outer surface protein C (ospC) knockout, PflaB-kan insertion in ospC (lp25−) | K. Tilly and P. A. Rosa, unpublished |
| Escherichia coli | ||
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| XL-10 Gold | Tetr Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac The F′ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr | Stratagene |
| BL21(DE3) pLysS | F−ompT hsdSB(rB− mB−) gal dcm λ(DE3) tonA pLysS (Camr) | Novagen |
| Plasmids | ||
| pET29b | Expression vector for protein purification | Novagen |
| pVsp1 | pBSV2::PflaB-vsp1 | 75 |
| pBSV2 | Shuttle vector (Kanr) | 67 |
| pKFSS1 | Shuttle vector (StrR) | 21 |
| pRJS1091 | pBSV2::PflaBospA22-mRFPΔ4 | This study |
| pRJS1090 | pBSV2::PflaBospA25-mRFPΔ4 | This study |
| pOSK240 | pBSV2::PflaBospC29-mRFPΔ4 | This study |
| pOSK258 | pBSV2::PflaBospC30-mRFPΔ4 | This study |
| pOSK257 | pBSV2::PflaBvsp1-31-mRFPΔ4 | This study |
| pOSK256 | pBSV2::PflaBvsp1-32-mRFPΔ4 | This study |
| pOSK200 | pKFSS1::PflaB-ospC | This study |
| pOSK273 | pKFSS1::PflaBospC(ΔN20-A30) | This study |
| pOSK274 | pKFSS1::PflaBospC(ΔN31-N41) | This study |
| pOSK299 | pKFSS1::PflaBospC(ΔS22) | This study |
| pOSK300 | pKFSS1::PflaBospC(ΔN21) | This study |
| pOSK287 | pKFSS1::PflaBospC(ΔN20-S22) | This study |
| pOSK288 | pKFSS1::PflaBospC(ΔG23-D25) | This study |
| pOSK301 | pKFSS1::PflaBospC(ΔN21-S22) | This study |
| pOSK294 | pKFSS1::PflaBospC(ΔA33-N41) | This study |
| pOSK302 | pKFSS1::PflaBospC(ΔD34-N41) | This study |
| pOSK309 | pKFSS1::PflaBospC(Ala)21-22 | This study |
| pOSK310 | pKFSS1::PflaBospC(Gly)21-22 | This study |
| pOSK268 | pBSV2::PflaBvsp1(ΔN20-S25) | This study |
| pOSK269 | pBSV2::PflaBvsp1(ΔG23-D28) | This study |
| pOSK262 | pBSV2::PflaBvsp1(ΔN20-S22) | This study |
| pOSK263 | pBSV2::PflaBvsp1(ΔG23-S25) | This study |
| pOSK275 | pBSV2::PflaBvsp1(ΔN20-A32) | This study |
| pOSK276 | pBSV2::PflaBvsp1(ΔK33-I39) | This study |
| pOSK279 | pBSV2::PflaBvsp1(ΔN21-S25) | This study |
| pOSK278 | pBSV2::PflaBvsp1(ΔS22-S25) | This study |
| pOSK284 | pBSV2::PflaBvsp1(ΔN20-G23) | This study |
| pOSK285 | pBSV2::PflaBvsp1(ΔN20-T24) | This study |
| pOSK289 | pBSV2::PflaBvsp1(ΔS22-S25) | This study |
| pOSK291 | pBSV2::PflaBvsp1(ΔN21-G23) | This study |
| pOSK292 | pBSV2::PflaBvsp1(ΔS22-T24) | This study |
| pOSK313 | pBSV2::PflaBvsp1(ΔN20-S25)P26A | This study |
| pOSK351 | pET29b::pT7ospCN20-His | This study |
| pOSK352 | pET29b::pT7ospCN31-His | This study |
| pOSK353 | pET29b::pT7ospCV37-His | This study |
| pOSK248 | pBSV2::PflaB-vsp1D60K/D87K/D150K | This study |
| pOSK307 | pKFSS1::PflaB-ospC-linker-his tag | This study |
| pOSK312 | pKFSS1::PflaB-ospC-(ΔS22)-linker-His tag | This study |