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. 2011 Jun;193(11):2861–2867. doi: 10.1128/JB.01552-10

Fig. 2.

Fig. 2.

Binding assays for SsoHflX with purified 50S ribosomal subunits in the presence of different guanine nucleotides. (A to C) A 1 μM concentration of recombinant SsoHflX was mixed with 1 μM 50S ribosomal subunit in the presence of 100 μM GppNHp (A) or 100 μM GDP (B) and in the absence of nucleotides (C). (D) Binding assay carried out as described above with the C-terminal G domain deletion mutant protein SsoHflX-H in the absence of nucleotides. (E and F) Migration of SsoHflX (E) and SsoHflX-H (F) in the absence of 50S ribosomal subunits as detected by silver staining.