Fig. 3.

Examination of SsoHflX by NMR spectroscopy. (A) 2-D ¹H-¹⁵N SOFAST-HMQC of SsoHflX at 50°C; (B) pulsed-field gradient diffusion NMR characterization of SsoHfIX (diamonds) compared to the 50S ribosomal subunit (open circles) at 25°C. The logarithm (y axis) of normalized resonance intensity (I/I₀) is plotted against G², the square of the gradient strength (x axis). I₀ is the intensity at low gradient strength. The diffusion profile of SsoHflX is homogeneous, as indicated by the linear regression of ln(I/I₀) as a function of G². The diffusion coefficient extracted from the slope of ln(I/I₀) as a function of G² is 1.1 × 10⁻¹⁰ m² s⁻¹. The diffusion profile of the 50S resonances also appears to be homogeneous and corresponds to a diffusion coefficient of 4.2 × 10⁻¹¹ m² s⁻¹. Error bars indicate the uncertainty of the signal intensity based on the spectral noise.