Fig. 5.
Basal nucleotidase activity of SsoHflX. Samples were incubated at 50°C for 20 min with the indicated concentrations of GTP. For all samples, GTP hydrolysis was corrected for background occurring in the absence of SsoHflX under otherwise identical conditions. (A) Substrate concentration-dependent GTPase activity of SsoHflX. (B) Time course experiment to test for ATPase activity of SsoHflX. Samples contained 7.5 μM SsoHflX and 100 μM ATP (filled circles) or GTP (open circles). NTP, nucleoside triphosphate. Error bars show 1 standard deviation.