Table 1.
The CRISPR/Cas cluster comprises a LeuO-dependent operon in S. Typhia
| Plasmid | CAT sp act (μmol/min/mg protein) |
|
|---|---|---|
| IMSS-1/pFMTrc12 | IMSS-1/pFMTrcleuO-50 | |
| pKK9-RR-casA-cas2 | 36 ± 3 | 273 ± 5 |
| pKK9-ΔRR-casA-cas2 | 0 | 0 |
| pKK9-RR-casA-CRISPR | 8 ± 11 | 137 ± 0.6 |
a
Transcriptional values for the LeuO-dependent casABCDE12-CRISPR operon were determined in MA medium. The plasmids pKK9-RR-casA-cas2, pKK9-ΔRR-casA-cas2, and pKK9-RR-casA-CRISPR were introduced into IMSS-1 harboring plasmid pFMTrc12 and IMSS-1 containing plasmid pFMTrcleuO-50. The CAT specific activity of each strain was determined using samples collected at OD595 values of 0.4, 0.6, 0.8, and 1 and at 12 h; the values presented are the results obtained at 12 h. IPTG (50 μM) was used to induce the pFMTrcleuO-50 plasmid. Data are means ± standard deviations for three independent experiments performed in duplicate.