Fig. 2.

Determination of the transcription start site of the lcfA-fadRB-etfBA operon by primer extension analysis. Total RNA of strain FU988 [PlcfA(−cre)-lacZ; the ΔfadR mutant] (lane 1) was prepared and used for the reverse transcription reaction to generate runoff cDNA. Lanes A, T, G, and C contained the products of the dideoxy sequencing reactions obtained with the same primers as those used for reverse transcription; the open arrowhead indicates a false band observed in the A, T, G, and C lanes. The runoff cDNA is indicated by an arrow. The partial nucleotide sequence of the coding strand corresponding to the ladders is shown; the −35 and −10 sequences are underlined, and the transcription start site (+1) is enclosed in a box.