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. 2011 May;193(10):2388–2395. doi: 10.1128/JB.00016-11

Fig. 5.

Fig. 5.

Examination of lacZ expression under the control of each of the lcfA-fadRB-etfBA, lcfB, and fadNAE promoters (PlcfA, PlcfB, and PfadN) in the ΔfadR and (or) ΔccpA backgrounds, and deletion analysis of the cre-lcfA, cre-lcfB, and cre-fadN sequences. The lcfA, lcfB, and fadN promoter regions with and without the cre were placed upstream of lacZ, and each of the resultant lacZ fusions (PlcfA-lacZ, PlcfB-lacZ, and PfadN-lacZ with and without the cre) was integrated into the amyE loci of the wild-type, ΔfadR, ΔccpA, and ΔfadR ΔccpA strains. (A) lacZ expression under the control of each of the promoters was monitored during growth with and without Glc, as described in the text, using PlcfA-lacZ strains FU989 (wild type, +cre) (columns 1 and 2, −/+ Glc), FU987 (wild type, −cre) (3 and 4, −/+ Glc), FU1071 (the ΔccpA mutant, +cre) (5 and 6, −/+ Glc), FU990 (the ΔfadR mutant, +cre) (7 and 8, −/+ Glc), FU988 (the ΔfadR mutant, −cre) (9 and 10, −/+Glc), and FU1048 (the ΔfadR ΔccpA mutant) (11 and 12, −/+ Glc). PlcfB-lacZ strains (B), FU961 (wild type, +cre), FU960 (wild type, −cre), FU1074 (the ΔccpA mutant, +cre), FU964 (the ΔfadR mutant, +cre), FU962 (the ΔfadR mutant, −cre), and FU965 (the ΔfadR ΔccpA mutant), and PfadN-lacZ strains (C), FU993 (wild type, +cre), FU991 (wild type, −cre), FU1051 (the ΔccpA mutant, +cre), FU994 (the ΔfadR mutant, +cre), FU992 (the ΔfadR mutant, −cre), and FU1053 (the ΔfadR ΔccpA mutant), were also used; the column assignments for PlcfB-lacZ and PfadN-lacZ are the same as those for the PlcfA-lacZ strains. The average β-Gal activities at an OD600 of 1, with standard deviations, which were obtained in two independent lacZ monitoring experiments, are shown. In the case that the LacZ activity was less than 2 nmol/min per OD, the maximal activity in the y axis is also set to 2 nmol/min per OD. The glucose-repression ratios of each strain are shown on bold gray bars. Significant differences between the average β-Gal activities are shown by ** (P < 0.05) and * (0.05 < P < 0.15) (Student's t test).