. 2011 May;193(10):2652–2656. doi: 10.1128/JB.01530-10

Table 1.

Oligonucleotide primers used in this study

Primer	Description/function^a	Sequence (5′-3′)^b
P1	flgB promoter (F)	GGATCCTAATACCCGAGCTTCAAG
P2	flgB promoter (R)	CATATGACCTCCCTCATTTAAAATTGC
P3	MCP3 gene-gfp fusion (F)	CATATGACAGATGAGAATTTAATTGATG
P4	MCP3 gene-gfp fusion (R)	TCGCGAAAGAATATCTTTAATCTCATC
P5	gfp (F) plus 5× glycine	TCGCGAACCTCCACCTCCACCAGTAAAGGAGAAGAACTTTTCACT
P6	gfp (R)	CTGCAGTTTGTATAGTTCATCCATGCCATGTG
P7	MCP5 gene-gfp fusion (F)	CATATGGTTAGTATGAAGCTTAAAGC
P8	MCP5 gene-gfp fusion (R)	TCGCGATTCGATCTTAAAATAATCAACAG
P9	MCP3 overexpression (F)	AGATCTTTTTTAAACAGTGTGTCTGC
P10	MCP3 overexpression (R)	CTGCAGAGAATCTCTAACTGGAAAAC
P11	MCP5 overexpression (F)	AGATCTTCAATGGAAGAGAAAGTTAG
P12	MCP5 overexpression (R)	CTGCAGATTAATCCCTCTAAAAGACC

F, forward; R, reverse.

The underlined sequences are the engineered restriction cut sites for DNA cloning, and the boldface sequence (P5) is the sequence encoding a five-glycine linker (34) at the 5′ end of gfp (9).