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. 2011 May;193(10):2619–2629. doi: 10.1128/JB.00120-11

Fig. 7.

Fig. 7.

Immunoblot analysis of HetR. Partially purified proteins were probed with penta-His antibody (Qiagen) (lower panel) to detect HetR-6His in AMC1761 (ΔhetR/hetR-6His) and AMC1766 (ΔhetR/hetR-6His/pknE-OE), which overexpresses pknE. Partially purified proteins from UHM103 (ΔhetR) and AMC1566 (rpoB-6His) were used as controls. The samples were resolved on 12% SDS-PAGE gels, and the proteins were stained with Coomassie brilliant blue G-250 (upper panel). Lane M contains the PageRuler prestained protein ladder size marker (Fermentas). Asterisks in the Coomassie blue-stained gel mark the sizes of the bands detected in the immunoblot.