Table 1.
Anabaena sp. strain PCC 7120 and E. coli strains used in this study
| Strain | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Anabaena PCC 7120 | ||
| AMC236 | ntcA::Ω Spr/Smr cassette | 32 |
| AMC451 | ΔpatS::Ω Spr/Smr cassette | 32 |
| AMC484 | WTa carrying shuttle plasmid pAM1951 (PpatS-gfp) | 32 |
| AMC485 | WT carrying shuttle plasmid pAM1956 (promoterless gfp) | 32 |
| AMC1256 | WT carrying shuttle plasmid pAM3264 (PhetR-gfp) | 30 |
| AMC1289 | WT hetR replaced by hetRR223W mutant allele | 15 |
| AMC1535 | WT Anabaena sp. strain PCC 7120 | R. Haselkorn |
| AMC1552 | WT carrying shuttle plasmid pAM3853 (PpknE-P-gfp) | This study |
| AMC1553 | WT carrying shuttle plasmid pAM3855 (PpknE-P1-gfp) | This study |
| AMC1554 | WT carrying shuttle plasmid pAM3857 (PpknE-P2-gfp) | This study |
| AMC1555 | WT carrying shuttle plasmid pAM3859 (PpknE-P3-gfp) | This study |
| AMC1556 | AMC236 carrying shuttle plasmid pAM3857 (PpknE-P2-gfp) | This study |
| AMC1557 | AMC236 carrying shuttle plasmid pAM3859 (PpknE-P3-gfp) | This study |
| AMC1558 | UHM103 carrying shuttle plasmid pAM3857 (PpknE-P2-gfp) | This study |
| AMC1559 | UHM103 carrying shuttle plasmid pAM3859 (PpknE-P3-gfp) | This study |
| AMC1560 | WT carrying shuttle plasmid pAM3826 (Pall2334-gfp) | This study |
| AMC1561 | WT carrying shuttle plasmid pAM3828 (PpknE-gfp) | This study |
| AMC1562 | WT carrying shuttle plasmid pAM3830 (Pall4668-gfp) | This study |
| AMC1563 | WT carrying shuttle plasmid pAM3832 (Pall4838-gfp) | This study |
| AMC1566 | WT carrying shuttle plasmid pAM4266 (rpoB-6His) | This study |
| AMC1702 | AMC236 carrying shuttle plasmid pAM1956 (promoterless gfp) | This study |
| AMC1704 | UHM103 carrying shuttle plasmid pAM1956 (promoterless gfp) | This study |
| AMC1759 | WT carrying shuttle vector pAM3919 (PpknE-PmutntcA-gfp) | This study |
| AMC1760 | WT carrying shuttle vector pAM3920 (PpknE-P2mutntcA-gfp) | This study |
| AMC1761 | UHM103 carrying shuttle vector pAM4375 (native-promoter-driven hetR with C-terminal 6His tag) | This study |
| AMC1762 | AMC1761 carrying pAM4012 (pAM1956-pknE-P with Ω Spr/Smr) | This study |
| AMC1763 | Knockout of pknE by insertion of pAM2178 through homologous single recombination in WT strain | This study |
| AMC1764 | WT carrying pAM4378 (PpetE-pknE) | This study |
| AMC1765 | WT carrying pAM4146 (native-promoter-driven pknE) | This study |
| AMC1766 | AMC1761 carrying pAM4171 (native-promoter-driven pknE) | This study |
| AMC1767 | AMC451 carrying pAM4146 (native-promoter-driven pknE) | This study |
| AMC1768 | AMC1289 carrying pAM4146 (native-promoter-driven pknE) | This study |
| AMC1769 | WT carrying shuttle plasmids pAM3264 (PhetR-gfp) and pAM4171 (native-promoter-driven pknE) | This study |
| AMC1770 | WT carrying shuttle plasmids pAM1951 (PpatS-gfp) and pAM4171 (native-promoter-driven pknE) | This study |
| AMC1771 | WT carrying pAM3318 (PpetE-hetRR223W) | This study |
| AMC1772 | AMC1771 carrying pAM4146 (native-promoter-driven pknE) | This study |
| AMC1780 | AMC1763 carrying shuttle plasmid pAM3264 (PhetR-gfp) | This study |
| UHM103 | ΔhetR; Het− (also AMC1537) | 1 |
| E. coli | ||
| AM1359 | DH10B carrying pRL623 and pRL443, for biparental conjugations with Anabaena | 32 |
| AM3626 | BL21(DE3) carrying pGEX-4T1-hetR, for IPTG-inducible expression of GST-HetR | 27 |
| AM3883 | BL21(DE3) carrying pQE30-2339, for IPTG-inducible expression of His-HetR | This study |
| AM3924 | AM4361 carrying pAM1951 (PpatS-gfp), for in vivo HetR activity assay, positive control | This study |
| AM3925 | AM3883 carrying pAM1951 (PpatS-gfp), for in vivo HetR activity assay, positive control | This study |
| AM3926 | AM4361 carrying pAM3857 (PpknE-P2-gfp), for in vivo assay | This study |
| AM3927 | AM3883 carrying pAM3857 (PpknE-P2-gfp), for in vivo assay | This study |
| AM4156 | AM4361 carrying pAM1954 (PrbcL-gfp), for in vivo assay, negative control | This study |
| AM4158 | AM3883 carrying pAM1954 (PrbcL-gfp), for in vivo assay, negative control | This study |
| AM4361 | BL21(DE3) carrying pQE30 vector alone, as negative-control strain for HetR activity | This study |
| AM4370 | BL21(DE3) carrying pGEX-4T1 vector alone, source of negative-control protein GST | This study |
| BL21(DE3) | Expression host and in vivo assay host | Invitrogen |
| DH10B | Cloning host | Invitrogen |
| GeneHogs | Cloning host | Invitrogen |
a
WT, wild type.