Table 2.

Plasmids used in this study

Plasmids	Relevant characteristicsa	Primers usedb	Reference(s) or source
pLT06	Integrational vector, contains P-pheS*; Cmr		24, 37
pRR17Δ	pLT06 derivative containing flanking regions of RR17: upstream, 663-bp region including 84 bp of the 5′ end of RR17; downstream, 532-bp region including 52 bp of the 3′ end of RR17	Upstream, RR17del5′BamHI, RR17del5′ overlap; downstream, RR17del 3′overlap, RR17del3′SalI	This study
pHK17Δ	pLT06 derivative containing flanking regions of HK17: upstream, 779-bp region including 105 bp of the 5′ end of HK17; downstream, 786-bp region including 57 bp of the 3′ end of HK17	Upstream, RR17Bam25′, HK17del5′overlap; downstream, HK17del3′SalI, HK17del3′ overlap	This study
pRhoΔ	pLT06 derivative containing flanking regions of Rho: upstream, 689-bp region including 45 bp of the 5′ end of Rho; downstream, 788-bp region including 51 bp of the 3′ end of Rho	Upstream, Rhodel5′BamHI, Rhodel5′overlap; downstream, Rhodel 3′overlap, Rhodel3′PstI	This study
pML28	pAT28 containing the aphA-3 promoter		18
pML28-RR17	pML28 derivative containing the RR17 sequence		11
pUC19	High-copy-number cloning vector; Ampr		44
pTCVlacSpec	lacZ transcriptional fusion vector; Specr		11
pTCV-RR17p	pTCVlacSpec derivative; contains nt −722 to +122 of RR17		11
pP2	pTCVlacSpec derivative; contains nt −659 to +109 of eutG	EutTEco5′, EutGBam3′	This study
pSP	pTCVlacSpec derivative; contains nt −248 to +109 of eutG	EutTGEco5′, EutGBam3′	This study
pSP2	pTCVlacSpec derivative; contains nt −659 to −254 of eutG	EutTEco5′, EutTGBam3′	This study
pSP3	pTCVlacSpec derivative; contains nt −659 to −296 of eutG	EutTEco5′, EutTG2Bam	This study
pSP4	pTCVlacSpec derivative; contains nt −659 to −139 of eutG	EutTEco5′, EutTG3Bam	This study
pSP6	pTCVlacSpec derivative; contains nt −248 to −84 of eutG	EutTGEco5′, EutTG4Bam	This study

^ant, nucleotides.

^bSequences of primers are reported in Table 3.