Fig. 8.

Effects of CsgD on the csgD promoter. (A) E. coli BWWF1D (white bars) and BWcsgDF1D (gray bars) were grown in YESCA medium for 6 (lanes 1 and 2), 9 (lanes 3 and 4), or 24 h (lanes 7 and 8) for measurement of β-galactosidase activities. (B) E. coli BWcsgDF1D was transformed with either pBAD18 (lane 1) or pBADcsgD (lane 2) and grown in YESCA medium at 28°C for 12 h, and then β-galactosidase activities were measured. (C) Confirmation of CsgD expression in pBADcsgD-transformed E. coli BWcsgDF1D by Western blot analysis. (D) Locations of CsgD-binding sites along the csgD promoter. The binding sites of other transcription factors on the cagD promoter have been reported previously (36, 37).