Fig. 1.

Cytotoxicity and cytolysin/hemolysin activity of V. vulnificus luxO and smcR mutants as well as their complemented strains cocultured with HEp-2 cells. The cytotoxicity (A) and cytolysin/hemolysin activity (B) in the conditioned medium were determined after coculture at an MOI of 10 for 4 h. ::psmcR, complementation with smcR. The bars represent means ± standard deviations; n = 3. Bars showing no significant difference are labeled with the same letters, and those showing significant difference (P < 0.05) are labeled with different letters based on one-way ANOVA with Tukey's HSD test.