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DNase I footprinting for determining the LitR binding site. The assay was performed on the sense (+) and antisense (−) strands. The amounts of recombinant LitR protein added to the reaction are shown. The position numbering is based on that for PcrtB (the transcriptional start point of PcrtB is numbered +1). The DNase I digests were run with the same probes that were chemically cleaved (G+A and T+C lanes).
