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. 2011 May;193(10):2536–2548. doi: 10.1128/JB.00815-10

Fig. 10.

Fig. 10.

Lrp stimulates cadBA expression. (A) Effect of lrp mutation on the expression of the cadBA operon under different growth conditions. Strains MG-CR (PcadBA::lacZ ΔcadBA) and MG-CR15 (PcadBA::lacZ Δlrp::Kmr ΔcadBA) were grown in glucose minimal medium at pH 7.6 or 5.8 with or without the addition of 5 mM lysine (lys.) under microaerobic conditions. After 7 h of incubation, samples were collected and β-galactosidase activities were determined. (B) Complementation of the Δlrp::Kmr mutant with the pET16lrp plasmid. Strains MG-CR/pET16b, MG-CR15/pET16b, and MG-CR15/pET16lrp were cultivated in glucose minimal medium (pH 5.8) with 5 mM lysine. After 7 h of incubation, β-galactosidase activities were determined. (C) Binding of His6-Lrp to the PcadBA promoter/control region. A fragment encompassing the PcadBA promoter/control region was incubated with increasing concentrations of purified His6-Lrp in the presence of salmon sperm DNA as a nonspecific competitor. The positions of free DNA (F) and the Lrp-DNA complex (B) are marked.