Fig. 5.

Modeling of G272R in the pKM101 crystal structures. (A) Ribbon model of VirB10 (TraF) showing the β-barrel (in gray) and antenna projection (AP) (green). The G272R mutation sits in an unstructured domain between the β-barrel and AP. (B) Space-filling model of the pKM101 core complex comprised of homologs of VirB7 (TraN) (pink), VirB9 (TraO) (blue), TraF (VirB10) β-barrel/linker domains (gray), and AP domain (α2, α3 helices, and intervening loop) (green). VirB10 G272R, corresponding to the TraF G294R mutation, is indicated in red. The AP domain spans residues 285 to 334 and the Δ308-337 mutation encompassing the AP loop and α3 helix confers a Tra⁺ Pil⁻ phenotype (26).