Table 2.
Effects of G272R mutation on DNA transfer
| Virulence on Kalanchoea | Donor strainb (+ pML122Genr) | Recipient strain | Transfer frequency (no. of transconjugants/donor)c |
|---|---|---|---|
| +++ | A348 | A348Spcr | 2.2 (±0.6) × 10−5 |
| − | PC1010 | A348Spcr | <10−8 |
| ++ | PC1010(pLB1310) | A348Spcr | 9.3 (±0.8) × 10−6 |
| ++ | PC1010(pLB1311) | A348Spcr | 9.7 (±0.4) × 10−6 |
| A348 | PC1010Spcr(pLB1311) | 2.3 (±1.3) × 10−5 | |
| PC1010 | PC1010Spcr(pLB1311) | <10−8 | |
| PC1010(pLB1310) | PC1010Spcr(pLB1311) | 1.1 (±1.5) × 10−5 | |
| A348 | PC1010Spcr | 4.8 (±0.6) × 10−8 | |
| A348 | PC1010Spcr(pLB1310) | 3.5 (±0.4) × 10−5 |
a
Inoculated K. daigremontiana leaves were scored for tumor formation on a scale of no tumors (−) to tumors incited by WT A348 (+++). Results are for strains without the IncQ plasmid.
b
A348, WT; PC1010, ΔB10. pLB1310 produces native VirB10, and pLB1311 produces VirB10.G272R.
c
Data are the means of triplicate determinations (± standard deviation) from a single experiment and are representative of three or more independent assays.