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Cysteine-dependent oligomerization. CidA-H and LrgA-H were expressed in E. coli and analyzed by Western blotting. Whole-cell lysates of induced E. coli C43 cells expressing LrgA-H (lane 3), LrgA-H(C55S C114S) (lane 4), CidA-H (lane 5), and CidA-H(C104S) (lane 6). Lane 1 contains size markers, with molecular masses (in kilodaltons) indicated to the left of the blots, and lane 2 contains proteins from the vector-only control.
