Table 2.
Primers used in this study
| Primer name | Primer sequencea |
|---|---|
| lrgA-BamHI-F | 5′-CCGGGGATCCATCAAACGTAGGAGGCAATG-3′ |
| cidA-BamHI-F | 5′-GCAGGATCCATATTTAGAAAGGGATGGCGCCATGCA-3′ |
| agrB-TIR-BamHI-F | 5′-GCGGATCCTGATTAACTTTATAAGGAGGAAAAACATATGTCGTATAATGACAG-3′ |
| lrgA-sGFP-link-R | 5′-GTTCTTCTCCTTTGCTATCATGAGCTTGTGCC-3′ |
| cidA-sGFP-link-R | 5′-GTTCTTCTCCTTTGCTTTCATAAGCGTCTACAC-3′ |
| agrB-sGFP-link-R | 5′-GTTCTTCTCCTTTGCTTTTTAAGTCCTCCTTAATAAAG-3′ |
| sGFP-TIR-BamHI-F | 5′-GGCGGATCCTGATTAACTTTATAAGGAGGAAAAACATATGAGCAAAGGAGAAGAAC-3′ |
| sGFP-EcoRI-R | 5′-GGCGAATTCTTATTTGTAGAGCTCATCCATGCC-3′ |
| lrgA-NdeI-F | 5′-GCGGCATATGGTCGTGAAACAACAAAAAGACGC-3′ |
| cidA-NdeI-F | 5′-GCGGCATATGCACAAAGTCCAATTAATAATCAAAC-3′ |
| lrgA-XhoI-R | 5′-CCGCTCGAGATCATGAGCTTGTCG-3′ |
| cidA-XhoI-R | 5′-CCGCTCGAGTTCATAAGCGTCTAC-3′ |
| lrgA-G164C-R | 5′-CAGCACCAGTAGATAATAATACAAATAATA-3′ |
| lrgA-G164C-F | 5′-ATTATTATCTACTGGTGCTGTTAAGTTAGG-3′ |
| lrgA-G341C-R | 5′-AGCCAGTAGAAATAAGTAATAGTATTGTTG-3′ |
| lrgA-G341C-F | 5′-ATTACTTATTTCTACTGGCTATGTCACACA-3′ |
| cidA-G311C-R | 5′-AACGATAGATGTTCCTATGATAATG-3′ |
| cidA-G311C-F | 5′-AACATCTATCGTTGCATTATCTTC-3′ |
| cidA-up-EcoRI-F | 5′-CGAATTCGAAGTCGTAAAGCAAGGAGGCATGAC-3′ |
| cidB-dn-SalI-R | 5′-CGTCGACGAAGCATTAGTTAAAGCATTACAAGCATGG-3′ |
a
Underlining indicates restriction endonuclease recognition sites used for subsequent cloning steps.