Fig. 2.

(A and B) HPAEC analyses of the glycogen side chains isolated from ΔmalP (A) and ΔglgA ΔmalP (B) derivatives of MC4100 after growth on maltose (G2) (A and B) or glucose (G1) (A). Note that the amount of glycogen in a ΔmalP strain grown on maltose is dramatically increased in comparison to that for growth on glucose. Under these conditions, the presence or absence of GlgA does not contribute to the amount of glycogen. (C) Spectral analysis of iodine-stained glycogen obtained from a ΔglgA ΔmalP strain, TBP38, grown on maltose (solid line) and of glycogen obtained from wild-type strain MC4100 grown on glucose (dashed line). Note that the sample of TBP38 had to be diluted to fall into the same absorption range as the sample of MC4100.