Fig. 4.

Alignment of sequence of T. guaymasensis ADH and other related zinc-containing ADHs. The sequences were aligned using Clustal W (64). Highlights in shadow, putative binding sites of catalytic zinc; highlights in box, putative motif of cofactor binding. TgADH, T. guaymasensis ADH; TbADH, T. brockii ADH; CbADH, C. beijerinckii ADH. Symbols indicate residues or nucleotides that are identical in all sequences in the alignment (*), conserved substitutions (:), semiconserved substitutions (.), or no corresponding amino acid (–).