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Purification of ADH from T. guaymasensis
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 3718.5 | 1.2 × 105 | 33.7 | 1 | 100 |
| DEAE-sepharose | 446.8 | 6.3 × 104 | 142 | 4.2 | 52 |
| Hydroxyapatite | 58.7 | 5.7 × 104 | 970 | 28.8 | 47 |
| Phenyl-sepharose | 30.7 | 3.4 × 104 | 1,099 | 32.6 | 28 |
| Gel filtration | 17.4 | 2.0 × 104 | 1,149 | 34.1 | 17 |
