Fig. 6.
Impact of sarA on phenol-soluble modulins. The PSM phenotype was assessed in stationary-phase cultures by HPLC as previously described (46). Strain designations: WT, wild-type strain; S, sarA mutant; SC, complemented sarA mutant; A, agr mutant; SA, sarA agr double mutant; SCA, sarA agr double mutant complemented for sarA. Results for each individual class (alpha- and beta-toxins versus delta-toxin) of PSM in both their formylated and unformylated forms were combined, and the amounts of each class observed in the USA300 isolate U1782 were set to 100%. Results observed in each of the indicated U1782 mutants and in U1 and its derivatives are shown relative to these amounts.