Fig. 9.

Impact of saeS on the sarA-dependent PSM phenotype of strain Newman. (A) The PSM phenotype was assessed by HPLC in Newman, its saeS-repaired [saeS(P18L)] derivative (P18L), and a Newman saeRS mutant. Results observed with strain Newman for each class of PSM were set to 100%, with results observed in each of the other strains shown relative to this amount. (B) The amount of SarA was assessed in Newman, its saeRS mutant, an saeS-repaired derivative (P18L), and an saeS(P18L) aureolysin mutant (P18Laur) by Western blotting as previously described (4).